Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: Primers and probes used for quantitative methylation-specific polymerase chain reaction (qMSP)

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Methylation, Polymerase Chain Reaction, Sequencing

Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: MGMT methylation frequencies and methylation level in methylated glioma samples

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Methylation, CpG Methylation Assay

Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: Overall survival in primary glioblastoma patients treated with standard radiotherapy and concomitant temozolomide . ( A ) Methylation status based on results from qMSP. Blue line; methylated, red line; unmethylated. ( B ) Methylation status based on results from pyrosequencing. Blue line; methylated, red line; unmethylated. ( C ) Methylation status based on both qMSP and pyrosequencing results. Blue line; methylated in both methods, red line; unmethylated in both methods, green line; methylated in pyrosequencing assay and unmethylated in qMSP assay. Abbreviation: qMSP, quantitative methylation specific PCR

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Methylation, Pyrosequencing Assay

Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: Patient characteristics and results of survival analysis for primary glioblastoma patients when stratified by MGMT methylation status

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Methylation, Standard Deviation

Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: Gene expression level of MGMT is associated with promoter DNA methylation status . MGMT gene expression in methylated (blue box plots) and unmethylated (red box plots) tissue samples analyzed by two different primer/probe sets (Assay 1; Hs00172470_m1 and assay 2; Hs01037698_m1). ( A ) Methylation status based on results from qMSP. ( B ) Methylation status based on results from pyrosequencing. Abbreviation: qMSP, quantitative methylation specific PCR

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Gene Expression, DNA Methylation Assay, Methylation

Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: Scatter plots of MGMT gene expression quantity in methylated and unmethylated samples . Red color indicates samples with negative methylation status by qMSP and pyrosequencing, blue color indicates samples with positive methylation status by qMSP and pyrosequencing, and green color indicate samples with non-concordant methylation status in the qMSP and pyrosequencing analysis. Circles indicate samples with loss of 10q26, whereas dots represent samples without loss of this region. Plots are based on the normalized gene expression detected by primer/probe set Hs00172470_m1. The plots based on the normalized gene expression detected by the other primer/probe set, Hs01037698_m1, were similar (data not shown). Abbreviation: qMSP, quantitative methylation specific PCR

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Gene Expression, Methylation

Journal: Clinical Epigenetics

Article Title: Genome- and epigenome-wide association study of hypertriglyceridemic waist in Mexican American families

doi: 10.1186/s13148-016-0173-x

Figure Lengend Snippet: Characteristics of the CpG sites significantly associated with HTGW

Article Snippet: Further, when we ran polygenic regression models and adjusted for the same covariates as mentioned in Model 1, Table , we found that the regression coefficient for pyrosequencing-based methylation at cg00574958 was −0.28 ( p = 4.4 × 10 −7 ).

Techniques: Methylation

Journal: Clinical Epigenetics

Article Title: Genome- and epigenome-wide association study of hypertriglyceridemic waist in Mexican American families

doi: 10.1186/s13148-016-0173-x

Figure Lengend Snippet: Improvement in Kullback-Leibler R 2 by including CpG sites significantly associated with HTGW a

Article Snippet: Further, when we ran polygenic regression models and adjusted for the same covariates as mentioned in Model 1, Table , we found that the regression coefficient for pyrosequencing-based methylation at cg00574958 was −0.28 ( p = 4.4 × 10 −7 ).

Techniques:

Journal: Clinical Epigenetics

Article Title: Genome- and epigenome-wide association study of hypertriglyceridemic waist in Mexican American families

doi: 10.1186/s13148-016-0173-x

Figure Lengend Snippet: Agreement between the results of HumanMethylation450 BeadChip array and pyrosequencing for the cg00574958 CpG site. a Correlation scatter plot. b Bland-Altman plot of the difference in the measurements of the two methods (ordinate) versus the mean (abscissa). Limits of agreement (LAG) are shown pictorially using dashed horizontal lines . Samples that fall outside the LAG are colored red . c Distribution of the DNA methylation as measured by pyrosequencing in individuals with ( red box ) and without ( yellow box ) HTGW

Article Snippet: Further, when we ran polygenic regression models and adjusted for the same covariates as mentioned in Model 1, Table , we found that the regression coefficient for pyrosequencing-based methylation at cg00574958 was −0.28 ( p = 4.4 × 10 −7 ).

Techniques: DNA Methylation Assay